Inflammatory gene profiles in gastric mucosa during Helicobacter pylori infection in humans

Détails

ID Serval
serval:BIB_1DA6F875F290
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Inflammatory gene profiles in gastric mucosa during Helicobacter pylori infection in humans
Périodique
Journal of Immunology
Auteur(s)
Wen  S., Felley  C. P., Bouzourene  H., Reimers  M., Michetti  P., Pan-Hammarstrom  Q.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
02/2004
Volume
172
Numéro
4
Pages
2595-606
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb 15
Résumé
Helicobacter pylori infection is associated with an inflammatory response in the gastric mucosa, ultimately leading to cellular hyperproliferation and malignant transformation. Hitherto, only expression of a single gene, or a limited number of genes, has been investigated in infected patients. cDNA arrays were therefore used to establish the global pattern of gene expression in gastric tissue of healthy subjects and of H. pylori-infected patients. Two main gene expression profiles were identified based on cluster analysis. The data obtained suggest a strong involvement of selected Toll-like receptors, adhesion molecules, chemokines, and ILs in the mucosal response. This pattern is clearly different from that observed using gastric epithelial cell lines infected in vitro with H. pylori. The presence of a "Helicobacter-infection signature," i.e., a set of genes that are up-regulated in biopsies from H. pylori-infected patients, could be derived from this analysis. The genotype of the bacteria (presence of genes encoding cytotoxin-associated Ag, vacuolating cytotoxin, and blood group Ag-binding adhesin) was analyzed by PCR and shown to be associated with differential expression of a subset of genes, but not the general gene expression pattern. The expression data of the array hybridization was confirmed by quantitative real-time PCR assays. Future studies may help identify gene expression patterns predictive of complications of the infection.
Mots-clé
Adhesins, Bacterial/genetics Adolescent Adult Age Factors Aged Antigens, Bacterial/genetics Bacterial Proteins/genetics Carrier Proteins/genetics Chronic Disease Female Gastric Mucosa/*immunology/microbiology/*pathology Gastritis/genetics/immunology/microbiology/pathology *Gene Expression Profiling/methods Genotype Helicobacter Infections/genetics/*immunology/microbiology/*pathology Helicobacter pylori/genetics/*immunology Humans Inflammation Mediators/analysis/*metabolism Male Middle Aged Oligonucleotide Array Sequence Analysis/methods Pyloric Antrum/immunology/microbiology/pathology Sex Factors
Pubmed
Web of science
Création de la notice
25/01/2008 16:58
Dernière modification de la notice
03/03/2018 14:34
Données d'usage