Economical microscope configuration for direct mycological examination with fluorescence in dermatology

Détails

ID Serval
serval:BIB_1D3A8D8F8E19
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Economical microscope configuration for direct mycological examination with fluorescence in dermatology
Périodique
Dermatology
Auteur(s)
Monod  M., Jaccoud  S., Stirnimann  R., Anex  R., Villa  F., Balmer  S., Panizzon  R.
ISSN
1018-8665 (Print)
Statut éditorial
Publié
Date de publication
2000
Volume
201
Numéro
3
Pages
246-8
Notes
Journal Article
Résumé
BACKGROUND: The use of fluorochromes such as Blankophor or Calcofluor allows to detect immediately and without ambiguity fungal elements in dermatological preparations. Whereas fluorescence microscopy is widely practised in clinical laboratories, it is not generally used in private practice because of the high price of a epifluorescence microscope. OBJECTIVE: To propose an economical microscope configuration to visualize fungal elements using fluorescence. METHODS: The preparations were examined with a standard microscope for routine observations, equipped with only two supplementary filters. RESULTS: Because the fungal elements produce a particularly bright fluorescence, a 25-watt halogen light is sufficient to visualize them in dermatological preparations: CONCLUSIONS: The proposed microscope configuration for direct mycological examination is particularly economical since equipment for epifluorescence and a vapour mercury lamp are not necessary.
Mots-clé
Dermatomycoses/*microbiology Humans Microscopy, Fluorescence/economics/instrumentation/*methods Microsporum/*cytology Trichophyton/*cytology
Pubmed
Web of science
Création de la notice
25/01/2008 16:54
Dernière modification de la notice
20/08/2019 12:53
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