Rheumatoid factor autoantibody-binding site: a molecular analysis using monoclonal antibodies with dual anti-TNP and anti-IgG activities

Détails

ID Serval
serval:BIB_1D0CDE3835A5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Rheumatoid factor autoantibody-binding site: a molecular analysis using monoclonal antibodies with dual anti-TNP and anti-IgG activities
Périodique
European Journal of Immunology
Auteur(s)
Reininger  L., Spertini  F., Shibata  T., Jaton  J. C., Izui  S.
ISSN
0014-2980 (Print)
Statut éditorial
Publié
Date de publication
11/1989
Volume
19
Numéro
11
Pages
2123-30
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Résumé
Two out of five murine IgG3 anti-trinitrophenyl (TNP) monoclonal antibodies (mAb) obtained either by immunization with TNP-keyhole limpet hemocyanin (KLH) (CB1, CB5, CB6 and 4H10) or with dinitrophenyl-lipopolysaccharide (9A6), exhibited anti-IgG rheumatoid factor (RF) activity (CB6 and 4H10). The anti-IgG activity of these two anti-TNP RF was specifically inhibited by murine IgG as well as by the hapten TNP. In order to identify the structural basis for the anti-IgG activity, the nucleotide sequences encoding the VH and VL regions were determined. By comparing the V regions of the non-RF and RF anti-TNP mAb, it was found that one anti-TNP RF antibody, CB6, shares virtually identical VL and VH regions with two anti-TNP antibodies, CB1 and CB5, but markedly differs from these in the D region. Furthermore, the light chain framework region 2 (FR2) and FR3 of non-RF mAb, CB1, CB5 and 9A6, have amino acid sequences almost identical to those claimed for anti-IgG1 RF activity (Shlomchik et al., J. Exp. Med. 1986. 164: 407). Our findings suggest, at least in the case of CB6 mAb, the involvement of CDR, but not light chain FR residues, in IgG binding.
Mots-clé
Amino Acid Sequence Animals Antibodies, Monoclonal/immunology Autoantibodies/genetics/*immunology Base Sequence Binding Sites, Antibody Immunoglobulin Heavy Chains/physiology Immunoglobulin Light Chains/physiology Immunoglobulin Variable Region/*genetics/physiology Mice Mice, Inbred Strains Molecular Sequence Data RNA, Messenger/genetics Rheumatoid Factor/genetics/*immunology Trinitrobenzenes/immunology
Pubmed
Web of science
Création de la notice
25/01/2008 16:20
Dernière modification de la notice
03/03/2018 14:33
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