Lymphocyte response to tetanus toxin T-cell epitopes: effects of tetanus vaccination and concurrent malaria prophylaxis

Détails

ID Serval
serval:BIB_1CF6B45F03FD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Lymphocyte response to tetanus toxin T-cell epitopes: effects of tetanus vaccination and concurrent malaria prophylaxis
Périodique
Vaccine
Auteur(s)
Fryauff  D. J., Mouzin  E., Church  L. W., Ratiwayanto  S., Hadiputranto  H., Sutamihardja  M. A., Widjaja  H., Corradin  G., Subianto  B., Hoffman  S. L.
ISSN
0264-410X (Print)
Statut éditorial
Publié
Date de publication
01/1999
Volume
17
Numéro
1
Pages
59-63
Notes
Clinical Trial
Journal Article
Randomized Controlled Trial
Research Support, U.S. Gov't, Non-P.H.S. --- Old month value: Jan
Résumé
Synthesized T-cell epitopes of tetanus toxin are universally immunogenic and serve to enhance immune response when they are used as vaccine carriers of B-cell epitopes. The immunogenicity of the P2, P30, and P2P30 T-cell epitopes of tetanus toxin and whole tetanus toxoid (TT) was evaluated by in vitro proliferation assay of lymphocytes from men with no history of tetanus vaccination who were enrolled in a malaria prophylaxis trial. The enhancement of immune response by tetanus vaccination (Td) and possible antagonism by the antimalarial drugs, was measured by pre- and post-Td comparisons within and between immunized prophylaxis groups (primaquine, chloroquine, placebo) and a nonimmunized control group. Constructs demonstrated low immunogenicity relative to TT in all groups. Relative to both control and its own baseline, the immunized primaquine prophylaxis group was distinct in demonstrating significantly increased proliferation against all three subunits and at both high (30 microg ml(-1)) and low (3 microg ml(-1)) concentrations. Immunization elicited significantly increased proliferation responses by placebo and chloroquine prophylaxis groups against only the P2P30 construct. Despite these significant post-Td changes, a low concentration of TT 0.1 microg ml(-1)) stimulated proliferation 7-10 times over that induced by the greatest concentration of the constructs.
Mots-clé
Adult Amino Acid Sequence Antimalarials/*therapeutic use Chloroquine/*therapeutic use Epitopes, T-Lymphocyte/*immunology Humans Lymphocyte Activation/*immunology Malaria/immunology/*prevention & control Male Molecular Sequence Data Placebos Primaquine/*therapeutic use T-Lymphocytes/immunology Tetanus Toxin/*immunology/pharmacology Tetanus Toxoid/*immunology/pharmacology
Pubmed
Web of science
Création de la notice
24/01/2008 15:55
Dernière modification de la notice
03/03/2018 14:33
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