Rapid detection of polyomavirus BK by a shell vial cell culture assay

Details

Serval ID
serval:BIB_1CA91078ACE1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Rapid detection of polyomavirus BK by a shell vial cell culture assay
Journal
Journal of Clinical Microbiology
Author(s)
Marshall  W. F., Telenti  A., Proper  J., Aksamit  A. J., Smith  T. F.
ISSN
0095-1137 (Print)
Publication state
Published
Issued date
07/1990
Volume
28
Number
7
Pages
1613-5
Notes
Journal Article --- Old month value: Jul
Abstract
Polyomavirus BK (BKV) causes asymptomatic latent infection in the human host that is reactivated during periods of immune suppression. Detection by conventional tube cell culture is difficult and time consuming because BKV exhibits slow growth with late (14 to 28 days) and subtle cytopathic effects. We developed a shell vial cell culture assay (SVA) using a cross-reactive monoclonal antibody to the T antigen of simian virus 40 to detect BKV rapidly by indirect immunofluorescence. Nuclear fluorescence was seen in BKV-infected cells as early as 16 h postinoculation; 6 to 28 times more foci were present at 36 h postinoculation. Human embryonic kidney cells infected with BKV produced 7 to 42 times more fluorescent foci than MRC-5 or rhabdomyosarcoma cells did. Centrifugation enhanced the infectivity of BKV in the SVA. To define the clinical utility of SVA, urine specimens from organ transplant patients were tested. Of 27 patients, 4 (15%) were found to be positive by SVA. SVA offers a simple and rapid method for detection of BKV that can be of use in clinical studies of this virus.
Keywords
Animals Antibodies, Monoclonal Antigens, Viral, Tumor/analysis BK Virus/immunology/*isolation & purification Cells, Cultured Cross Reactions Evaluation Studies Humans Polyomavirus/*isolation & purification Tumor Virus Infections/diagnosis Virology/*methods
Pubmed
Web of science
Create date
25/01/2008 14:45
Last modification date
20/08/2019 12:53
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