Ontogeny of alpha 1- and beta 1-isoforms of Na(+)-K(+)-ATPase in fetal distal rat lung epithelium

Détails

ID Serval
serval:BIB_1C73AE1515FD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Ontogeny of alpha 1- and beta 1-isoforms of Na(+)-K(+)-ATPase in fetal distal rat lung epithelium
Périodique
American Journal of Physiology
Auteur(s)
O'Brodovich  H., Staub  O., Rossier  B. C., Geering  K., Kraehenbuhl  J. P.
ISSN
0363-6143
Statut éditorial
Publié
Date de publication
05/1993
Volume
264
Numéro
5 Pt 1
Pages
C1137-43
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
Because immature, in contrast to mature, fetal lungs have ineffective Na transport, we wished to determine the ontogeny of Na(+)-K(+)-ATPase expression in fetal distal lung epithelium (FDLE). FDLE and fibroblasts (FLF) from 17- to 22-day gestational age fetal rats (term = 22 days) were grown in primary culture. Northern and slot-blot analyses utilizing isoform-specific cDNA probes determined that alpha 1- (3.7 kb) and beta 1- (2.7, 2.3, and 1.9 kb) transcripts were present in FDLE at levels approximately fivefold higher than in FLF. alpha 2-, alpha 3-, or beta 2-isoforms of Na(+)-K(+)-ATPase were not detected. In 17-day gestational age FDLE, only small amounts of alpha 1-mRNA levels were detectable, and there were approximately 10-fold less beta 1-isoform transcripts. By 20 days gestational age, the level of alpha 1-transcripts roughly doubled, whereas beta 1-levels increased approximately sixfold. Thus, during the transition from the canalicular to saccular stages of lung development, FDLE have a differentially regulated surge in mRNA levels of alpha 1- and beta 1-Na(+)-K(+)-ATPase isoforms and do not switch isoforms during lung development. Levels for both isoform transcripts then fell before birth, reaching values less than those seen for 17-day gestational age FDLE. FDLE vesicle Na(+)-K(+)-ATPase activity did not increase until 22 days gestational age.
Mots-clé
Animals Animals, Newborn Electrophoresis, Polyacrylamide Gel Epithelium/enzymology Fetus Fibroblasts/enzymology *Gene Expression Regulation, Enzymologic Gestational Age Isoenzymes/genetics/isolation & purification/*metabolism Lung/embryology/*enzymology Na(+)-K(+)-Exchanging ATPase/genetics/isolation & purification/*metabolism Poly A/analysis/metabolism RNA/genetics/isolation & purification RNA, Messenger/analysis/genetics/isolation & purification/metabolism Rats Rats, Wistar Transcription, Genetic
Pubmed
Web of science
Création de la notice
24/01/2008 13:28
Dernière modification de la notice
03/03/2018 14:32
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