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Efficiency of adeno-associated virus type-2 vectors in non-human primate Schwann cells.
Adult macaque Schwann cells were infected using adeno-associated virus type-2-derived vectors expressing the green fluorescent protein reporter gene under the control of the cytomegalovirus, the hybrid cytomegalovirus-betaactin, the myelin basic protein or the tetracycline-inducible promoters. On the basis of green fluorescent protein expression, gene transfer efficiency was compared in resting and dividing conditions following or not following hydroxyurea or etoposide treatment. Hydroxyurea allowed promoter-dependent expression of green fluorescent protein in infected Schwann cells. Etoposide treatment led to a high percentage of green fluorescent protein expressing cells (over 50%) with all promoters tested. When infected cells were grafted into demyelinated nude mice spinal cord, green fluorescent protein expression was only observed with the cytomegalovirus-betaactin and tetracycline-inducible promoters. In addition, adeno-associated virus type-2 infection reduced the grafted cell survival but increased their differentiation.
Analysis of Variance, Animals, Cell Count/methods, Cell Proliferation/drug effects, Cell Transplantation/methods, Cells, Cultured, Cytomegalovirus/physiology, Demyelinating Diseases/chemically induced, Demyelinating Diseases/surgery, Dependovirus/physiology, Etoposide/pharmacology, Fibroblasts/metabolism, Fibroblasts/virology, Gene Expression Regulation/drug effects, Gene Expression Regulation/physiology, Genetic Vectors/physiology, Green Fluorescent Proteins/metabolism, Hydroxyurea/pharmacology, Immunohistochemistry/methods, Macaca fascicularis, Mice, Myelin Basic Proteins/metabolism, Nucleic Acid Synthesis Inhibitors/pharmacology, Promoter Regions, Genetic/drug effects, Promoter Regions, Genetic/physiology, Schwann Cells/metabolism, Schwann Cells/virology, Time Factors, Transduction, Genetic
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