Pattern variations of polyclonal and monoclonal immunoglobulins of different isotypes analyzed by high-resolution two-dimensional electrophoresis

Details

Serval ID
serval:BIB_1C2FF223DF8E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Pattern variations of polyclonal and monoclonal immunoglobulins of different isotypes analyzed by high-resolution two-dimensional electrophoresis
Journal
Electrophoresis
Author(s)
Tissot  J. D., Hochstrasser  D. F., Spertini  F., Schifferli  J. A., Schneider  P.
ISSN
0173-0835 (Print)
Publication state
Published
Issued date
03/1993
Volume
14
Number
3
Pages
227-34
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Abstract
High-resolution two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) was used to analyze serum samples and purified immunoglobulins (Ig) obtained from "normal" individuals and from patients diagnosed with monoclonal gammopathies (MG) (n = 47; 5 IgA, 15 IgM, 15 IgG, 4 biclonal IgG, 1 IgD, 7 Bence Jones proteins). Polyclonal and monoclonal heavy (H) chains were located at different restricted gel positions according to their isotype. Monoclonal H chains appeared as sets of spots characterized by charge (pI) and size (M(r)) microheterogeneity. Most of the monoclonal gamma chains were not seen on the gels (12/15). Supplementary polypeptides of 45-48 kDa were detected in serum samples containing monoclonal IgM, but were not seen in MG of other isotypes. However, these polypeptides were not specifically associated with monoclonal IgM because they were also found on protein maps of purified polyclonal IgM. Polyclonal light (L) chains appeared as cloudy bands containing several zones of higher density, whereas monoclonal L chains were usually resolved as single sharp spots. In 6 samples, monoclonal L chains were not seen, and in 9 samples, they appeared as two or more spots, characterized by different pI and/or M(r). In one sample obtained from a patient with a biclonal gammopathy, the L chains were resolved as 4 different spots. Our results confirm that 2-D PAGE is an excellent tool to study Ig. Analysis of the L chain region of the gels was particularly informative. Several monoclonal L chains exhibited heterogeneous two-dimensional spot patterns, suggesting that "subtile" clonal mutations of B-cell lineage and/or posttranslational modifications were involved in their production.
Keywords
Antibodies, Monoclonal/*isolation & purification Blood Protein Electrophoresis/methods Electrophoresis, Gel, Two-Dimensional/*methods Evaluation Studies Humans Immunoglobulin Heavy Chains/isolation & purification Immunoglobulin Isotypes/*isolation & purification Immunoglobulin Light Chains/isolation & purification Immunoglobulin M/isolation & purification Paraproteinemias/immunology Peptide Mapping/methods
Pubmed
Web of science
Create date
25/01/2008 15:34
Last modification date
20/08/2019 12:52
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