Coupling ex vivo electroporation of mouse retinas and luciferase reporter assays to assess rod-specific promoter activity.

Détails

ID Serval
serval:BIB_1C18EAE8391C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Coupling ex vivo electroporation of mouse retinas and luciferase reporter assays to assess rod-specific promoter activity.
Périodique
Experimental Eye Research
Auteur(s)
Boulling A., Escher P.
ISSN
1096-0007 (Electronic)
ISSN-L
0014-4835
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
148
Pages
79-82
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
Ex vivo electroporation of mouse retinas is an established tool to modulate gene expression and to study cell type-specific gene expression. Here we coupled ex vivo electroporation to luciferase reporter assays to facilitate the study of rod-photoreceptor-specific gene promoters. The activity of the rod-specific proximal bovine rhodopsin promoter was significantly increased in C57BL/6J wild-type retinas at postnatal days 1 and 7 by 3.4-fold and 8.7-fold respectively. In C57BL/6J Nr2e3(rd7/rd7) retinas, where the rod photoreceptor-specific nuclear receptor Nr2e3 is not expressed, a significant increase by 2.5-fold was only observed at postnatal day 7. Cone-specific S-opsin promoter activity was not modulated in C57BL/6J wild-type and Nr2e3(rd7/rd7) retinas. Taken together, we describe an easily implementable protocol to assess rod-specific promoter activity in a physiological context resembling that of the developing postnatal mouse retina.
Pubmed
Web of science
Création de la notice
14/06/2016 18:07
Dernière modification de la notice
03/03/2018 14:31
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