Article: article from journal or magazin.
Relocalization of telomeric Ku and SIR proteins in response to DNA strand breaks in yeast.
Telomeric TG-rich repeats and their associated proteins protect the termini of eukaryotic chromosomes from end-to-end fusions. Associated with the cap structure at yeast telomeres is a subtelomeric domain of heterochromatin, containing the silent information regulator (SIR) complex. The Ku70/80 heterodimer (yKu) is associated both with the chromosome end and with subtelomeric chromatin. Surprisingly, both yKu and the chromatin-associated Rap1 and SIR proteins are released from telomeres in a RAD9-dependent response to DNA damage. yKu is recruited rapidly to double-strand cuts, while low levels of SIR proteins are detected near cleavage sites at later time points. Consistently, yKu- or SIR-deficient strains are hypersensitive to DNA-damaging agents. The release of yKu from telomeric chromatin may allow efficient scanning of the genome for DNA strand breaks.
Antigens, Nuclear, Bleomycin/pharmacology, Cell Cycle Proteins/metabolism, DNA Damage, DNA Helicases, DNA Repair, DNA-Binding Proteins/metabolism, Deoxyribonucleases, Type II Site-Specific/biosynthesis, Dimerization, Enzyme Induction, Fungal Proteins/metabolism, GTP-Binding Proteins/metabolism, Genotype, Nuclear Proteins/metabolism, Polymerase Chain Reaction, Saccharomyces cerevisiae/cytology, Saccharomyces cerevisiae/drug effects, Saccharomyces cerevisiae Proteins, Silent Information Regulator Proteins, Saccharomyces cerevisiae, Telomere/genetics, Telomere/physiology, Transcription Factors/metabolism, rap GTP-Binding Proteins
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