STEM tomography in cell biology.
Details
Serval ID
serval:BIB_1BEFAE29BA27
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
STEM tomography in cell biology.
Journal
Journal of Structural Biology
ISSN
1047-8477 (Print)
ISSN-L
1047-8477
Publication state
Published
Issued date
2007
Volume
159
Number
3
Pages
381-391
Language
english
Abstract
Transmission electron tomography has been used in biological sciences for quite some time and proven to be a valuable tool. However, to date, the different Scanning Transmission modes are almost not used for electron tomography on resin-embedded biological material. We explored different STEM modes on epon-embedded, osmium-uranyl-lead-stained biological material. Bright Field-TEM and High Angle Annular Dark Field-STEM tomograms from the same areas were recorded and compared. Contrast and signal-to-noise ratios were calculated. Template matching was used to validate results obtained in Bright Field-TEM and High Angle Annular Dark Field-STEM tomograms. It is concluded that High Angle Annular Dark Field-STEM gives a five times better contrast and signal-to-noise ratio than Bright Field-TEM. Template matching showed that 1.3 times more information could be extracted from High Angle Annular Dark Field-STEM tomograms than from Bright Field-TEM tomograms.
Keywords
Cell Membrane/ultrastructure, Cells/ultrastructure, Humans, Microscopy, Electron, Scanning Transmission/methods
Pubmed
Web of science
Create date
28/02/2012 19:55
Last modification date
20/08/2019 13:52