Objective To explore the feasibility of applying magnetic nanoparticle ferrofluid(MNPF) to postmortem magnetic resonance angiography(PMMRA). Methods MNPF of concentrations ranging from 0 to 1000μg/ml was prepared by modifying nanoparticle γ-Fe2 O3(30 nm) with oleic acid(OA) and then dispersing it in paraffin oil(PO). MNPF was transferred into tubes which were then sealed and properly fixed on the MR table. To acquire image data, a serial of MR sequences were employed, including T1 w-SE, T2 w-SE and 3 DT2*w-GR and characteristics of these images were analyzed. MNPF of γ-Fe2 O3 0μg/ml, 25μg/ml and 1000μg/ml was injected into ex situ bovine hearts, T1 w-SE and T2 w-SE sequences were used and characteristics of the acquired images were analyzed. Results When the concentration of γ-Fe2 O3 lies between 0μg/ml to 100μg/ml, MNPF has hyperintensities on T1 w images and hypointensities on T2 w images. On T2*w images, the signals of MNPF tend to decrease and then disappear when the concentration of γ-Fe2 O3 increases from 0μg/ml to 100μg/ml. When the concentration surpasses 100μg/ml, it may lead to the production of magnetic sensitive artifacts, which leads to improper application of MNPF to PMMRA. Conclusion Oily MNPF with a certain concentration range of γ-Fe2 O3 has the potential to be used as T1-positive and T2*-signal-adjustable contrast agent.