Pendrin: an apical Cl-/OH-/HCO3- exchanger in the kidney cortex.
Details
Serval ID
serval:BIB_1B97216D052C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Pendrin: an apical Cl-/OH-/HCO3- exchanger in the kidney cortex.
Journal
American journal of physiology. Renal physiology
ISSN
1931-857X (Print)
ISSN-L
1522-1466
Publication state
Published
Issued date
02/2001
Peer-reviewed
Oui
Volume
280
Number
2
Pages
F356-64
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Publication Status: ppublish
Abstract
The identities of the apical Cl-/base exchangers in kidney proximal tubule and cortical collecting duct (CCD) cells remain unknown. Pendrin (PDS), which is expressed at high levels in the thyroid and its mutation causes Pendred's syndrome, is shown to be an anion exchanger. We investigated the renal distribution of PDS and its function. Our results demonstrate that pendrin mRNA expression in the rat kidney is abundant and limited to the cortex. Proximal tubule suspensions isolated from kidney cortex were highly enriched in pendrin mRNA. Immunoblot analysis studies localized pendrin to cortical brush-border membranes. Nephron segment RT-PCR localized pendrin mRNA to proximal tubule and CCD. Expression studies in HEK-293 cells demonstrated that pendrin functions in the Cl-/OH-, Cl-/HCO3-, and Cl-/formate exchange modes. The conclusion is that pendrin is an apical Cl-/base exchanger in the kidney proximal tubule and CCD and mediates Cl-/OH-, Cl-/HCO3-, and Cl-/formate exchange.
Keywords
Analysis of Variance, Antiporters/metabolism, Carrier Proteins/metabolism, Chloride-Bicarbonate Antiporters, Humans, Kidney Cortex/metabolism, Kidney Medulla/metabolism, Kidney Tubules, Collecting/metabolism, Kidney Tubules, Proximal/metabolism, Membrane Transport Proteins, Molecular Sequence Data, RNA, Messenger/metabolism, Sulfate Transporters
Pubmed
Web of science
Create date
30/12/2020 15:04
Last modification date
31/12/2020 6:26
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