Chromosomal in situ suppression hybridization of immunologically classified mitotic cells in hematologic malignancies.

Details

Serval ID
serval:BIB_1B35B7DC15B1
Type
Article: article from journal or magazin.
Collection
Publications
Title
Chromosomal in situ suppression hybridization of immunologically classified mitotic cells in hematologic malignancies.
Journal
Genes, chromosomes & cancer
Author(s)
Tiainen M., Popp S., Parlier V., Emmerich P., Bellomo M.J., Ruutu T., Cremer T., Knuutila S.
ISSN
1045-2257
Publication state
Published
Issued date
1992
Volume
4
Number
2
Pages
135-40
Language
english
Abstract
Chromosomal in situ suppression (CISS) hybridization was performed with library DNA from sorted human chromosomes 8, 9, 15, 17, 21, and 22 on immunologically stained bone marrow cells of four patients with a hematologic neoplasm, including two patients with myelodysplastic syndrome and trisomy 8, one patient with promyelocytic leukemia bearing the translocation t(15;17)(q22;q11-12), and one patient with chronic myeloid leukemia and the translocation t(9;22)(q34;q11). In all patients, the results of conventional karyotype analysis could be confirmed by one- or two-color CISS hybridization using the appropriate chromosome-specific libraries. Our results show that CISS hybridization can detect both numerical and structural chromosome changes in immunologically classified cells with high specificity and reliability. The fact that chromosome spreads of very poor quality can now be included in such analyses is a decisive advantage of this approach. In addition, the suitability of this approach for interphase cytogenetics is discussed.
Keywords
Chromosome Aberrations, DNA Probes, Female, Genetic Techniques, Humans, Karyotyping, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, Leukemia, Promyelocytic, Acute, Male, Microscopy, Fluorescence, Mitosis, Myelodysplastic Syndromes, Nucleic Acid Hybridization
Pubmed
Create date
22/05/2009 9:25
Last modification date
20/08/2019 12:51
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