Heterogeneity and clonality among isolates of Mycobacterium kansasii: implications for epidemiological and pathogenicity studies

Details

Serval ID
serval:BIB_1B129C0931C2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Heterogeneity and clonality among isolates of Mycobacterium kansasii: implications for epidemiological and pathogenicity studies
Journal
Journal of Clinical Microbiology
Author(s)
Alcaide  F., Richter  I., Bernasconi  C., Springer  B., Hagenau  C., Schulze-Robbecke  R., Tortoli  E., Martin  R., Bottger  E. C., Telenti  A.
ISSN
0095-1137 (Print)
Publication state
Published
Issued date
08/1997
Volume
35
Number
8
Pages
1959-64
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Abstract
The reservoir and transmission route of Mycobacterium kansasii are largely unknown. In addition, culturing of M. kansasii from human sources is not proof of disease because it may represent colonization rather than infection. Unfortunately, investigation of the epidemiology and pathogenicity of M. kansasii is complicated by evidence of heterogeneity within the species. A comprehensive study by detailed genotypic analysis of a large collection of M. kansasii isolates (n = 276) from various geographical sources within Europe was conducted. Five defined subtypes of M. kansasii were identified; of these subtypes, type I represents the most common isolate from humans. Although phylogenetic analysis confirmed its relationship to the other M. kansasii types, significant sequence divergence was found at the 16S-23S intergenic spacer. Analysis of the chromosomal polymorphism of type I demonstrated a marked clonal structure for this particular organism. Because M. kansasii is becoming a significant pathogen among immunodeficient hosts, future epidemiological and pathogenicity studies should take into consideration both the heterogeneity within the species and the apparent clonality of the most prevalent M. kansasii isolates infecting humans.
Keywords
*Bacterial Proteins Base Sequence Chaperonins/*genetics DNA, Bacterial/*analysis Humans Molecular Sequence Data Mycobacterium/*classification/*genetics/isolation & purification Phylogeny Polymerase Chain Reaction Polymorphism, Restriction Fragment Length RNA, Bacterial/*analysis/genetics RNA, Ribosomal, 16S/*analysis/genetics Sequence Homology, Nucleic Acid Species Specificity
Pubmed
Web of science
Create date
25/01/2008 14:45
Last modification date
20/08/2019 12:51
Usage data