Article: article from journal or magazin.
DNA degradation in avian faecal samples and feasibility of non-invasive genetic studies applied to threatened capercaillie populations
We evaluated the feasibility of using faeces as a non-invasively collected DNA source for the genetic study of an endangered bird population (capercaillie; Tetrao urogallus). We used a multitube approach, and for our panel of 11 microsatellites genotyping reliability was estimated at 98% with five repetitions. Experiments showed that free DNases in faecal material were the major cause of DNA degradation. Our results demonstrate that using avian faeces as a source of DNA, reliable microsatellite genotyping can be obtained with a reasonable number of PCR replicates.
bird, DNA degradation, faeces, genotyping errors, microsatellites, non-invasive sampling
Web of science
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