Article: article from journal or magazin.
Detachment of cell surface-bound anticarcinoembryonic antigen immune complexes by phospholipase C.
CEA as well as normal cross-reacting antigens (NCA) are fixed to the cell membrane via phosphatidylinositol (PI). To find out whether these antigens are internalized after antibody contact, acid pH desorption was compared to phospholipase C (PLC)-mediated cleavage of the antigen anchor. With the former procedure, marked differences in the desorbability of individual MAbs were noted, while PLC was able to cleave off surface-bound immune complexes irrespective of the MAb involved. From this it is concluded that internalization of MAb complexes of CEA/NCA, if occurring at all, is a low efficiency process.
Antibodies, Monoclonal/immunology, Antigen-Antibody Complex/metabolism, Carcinoembryonic Antigen/immunology, Cell Line, Cell Membrane/immunology, Humans, Hydrogen-Ion Concentration, Phosphatidylinositols/metabolism, Tumor Cells, Cultured, Type C Phospholipases/metabolism
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