An activation-independent role of transcription factors in insulator function.

Détails

ID Serval
serval:BIB_1A97071D237A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
An activation-independent role of transcription factors in insulator function.
Périodique
EMBO Reports
Auteur(s)
Fourel G., Boscheron C., Revardel E., Lebrun E., Hu Y.F., Simmen K.C., Müller K., Li R., Mermod N., Gilson E.
ISSN
1469-221X[print], 1469-221X[linking]
Statut éditorial
Publié
Date de publication
02/2001
Volume
2
Numéro
2
Pages
124-132
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Chromatin insulators are defined as transcriptionally neutral elements that prevent negative or positive influence from extending across chromatin to a promoter. Here we show that yeast subtelomeric anti-silencing regions behave as boundaries to telomere-driven silencing and also allow discontinuous propagation of silent chromatin. These two facets of insulator activity, boundary and silencing discontinuity, can be recapitulated by tethering various transcription activation domains to tandem sites on DNA. Importantly, we show that these insulator activities do not involve direct transcriptional activation of the reporter promoter. These findings predict that certain promoters behave as insulators and partition genomes in functionally independent domains.
Mots-clé
Animals, Chromatin/genetics, Chromatin/metabolism, Chromosomes, Fungal/genetics, Chromosomes, Fungal/metabolism, DNA, Fungal/genetics, DNA, Fungal/metabolism, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/genetics, Fungal Proteins/chemistry, Fungal Proteins/genetics, Gene Expression Regulation, Fungal, Gene Silencing, Genes, Reporter, Promoter Regions, Genetic/genetics, Protein Structure, Tertiary, RNA, Messenger/biosynthesis, RNA, Messenger/genetics, Recombinant Fusion Proteins/chemistry, Recombinant Fusion Proteins/metabolism, Regulatory Sequences, Nucleic Acid/genetics, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins, Telomere/genetics, Telomere/metabolism, Transcription Factors/chemistry, Transcription Factors/genetics, Transcriptional Activation
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 10:41
Dernière modification de la notice
20/08/2019 12:51
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