Regulated expression of GLUT2 in diabetes studied in transplanted pancreatic beta cells.

Détails

ID Serval
serval:BIB_19429CB88950
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Regulated expression of GLUT2 in diabetes studied in transplanted pancreatic beta cells.
Périodique
Biochemical Society Transactions
Auteur(s)
Thorens B., Roduit R.
ISSN
0300-5127[print], 0300-5127[linking]
Statut éditorial
Publié
Date de publication
08/1994
Volume
22
Numéro
3
Pages
684-687
Langue
anglais
Notes
Publication types: Journal Article ; Review
Publication Status: ppublish
Résumé
GLUT2 disappearance is a marker of the beta cell glucose-unresponsiveness associated with diabetes. Understanding the factor(s) leading to this dysfunction may shed light on pathogenesis of diabetes. Since the regulation of GLUT2 expression in diabetes can so far only be studied in in vivo experiments, we developed a novel experimental approach to study the genetic regulation of GLUT2 in diabetes. By encapsulating islets or cell lines in semi-permeable membranes, these cells can be exposed to the diabetic environment of rats or mice and can be retrieved for analysis of GLUT2 expression and for the change in the secretory response to glucose. Immunocytochemical analysis of transporter expression reveals changes in protein expression while transcriptional analysis of GLUT2 gene expression could be performed in cells transfected with promoter-reporter gene constructs. Using this last approach we hope to be able to characterize the promoter regions involved in the beta cell- and diabetes-specific regulation of GLUT2 expression and possibly to determine which factors are responsible for this regulation.
Mots-clé
Animals, Biological Markers, Diabetes Mellitus, Experimental/genetics, Diabetes Mellitus, Experimental/metabolism, Diabetes Mellitus, Type 1/genetics, Diabetes Mellitus, Type 1/metabolism, Diabetes Mellitus, Type 2/genetics, Diabetes Mellitus, Type 2/metabolism, Gene Expression Regulation, Glucose Transporter Type 2, Islets of Langerhans/metabolism, Islets of Langerhans Transplantation/physiology, Mice, Monosaccharide Transport Proteins/genetics, Monosaccharide Transport Proteins/metabolism, Rats
Pubmed
Web of science
Création de la notice
24/01/2008 14:41
Dernière modification de la notice
03/03/2018 14:25
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