Sources of variation in patient response to radiation treatment

Details

Serval ID
serval:BIB_1896AF6C008F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Sources of variation in patient response to radiation treatment
Journal
International Journal of Radiation Oncology, Biology, Physics
Author(s)
Crompton  N. E., Shi  Y. Q., Emery  G. C., Wisser  L., Blattmann  H., Maier  A., Li  L., Schindler  D., Ozsahin  H., Ozsahin  M.
ISSN
0360-3016
Publication state
Published
Issued date
02/2001
Peer-reviewed
Oui
Volume
49
Number
2
Pages
547-54
Notes
Journal Article --- Old month value: Feb 1
Abstract
PURPOSE: To investigate sources of variation in radiosensitivity displayed by cancer patients and blood donors using the leukocyte apoptosis assay. METHODS AND MATERIALS: Probes were obtained from 105 healthy blood donors, 48 cancer patients displaying normal sensitivity to radiotherapy, 12 cancer patients displaying hypersensitivity to radiotherapy, 12 Ataxia telangiectasia blood donors, and 4 additional individuals with genetic diseases of potentially modified radiosensitivity; 2 neurofibromatosis (NF) donors, a Nijmegen breakage syndrome (NBS) donor, and an Immunodeficiency, Chromosome fragility, Facial anomaly syndrome (ICF) donor. Heparinized blood was diluted in medium, irradiated, and left to incubate for 48 h. CD4 and CD8 T-lymphocyte DNA was stained with propidium iodide and the cells were analyzed by flow cytometry. RESULTS: Radiation-induced apoptosis depended on age and cell type. Cohorts of hypersensitive cancer patients, NBS and AT donors displayed compromised apoptotic response. An asymmetric apoptotic response of T-lymphocytes was observed in an ICF donor and a cryptic hypersensitivity donor. Two NF donors displayed no abnormal sensitivity to radiotherapy but compromised apoptotic T-cell response to X-rays. CONCLUSION: Our studies reveal 4 physiologic sources of variation in radiation response-2 are genetic: cryptic hypersensitivity and hereditary disease, and 2 are epigenetic: cell type and donor age. They emphasize the important role of proteins involved in the recognition and repair of DNA double-strand breaks in determining the response of individuals to radiotherapy.
Keywords
Adolescent Adult Age Factors Aged Aged, 80 and over Apoptosis/*physiology Ataxia Telangiectasia/genetics Blood Donors CD4-Positive T-Lymphocytes/*radiation effects CD8-Positive T-Lymphocytes/*radiation effects Child Child, Preschool Cohort Studies Humans Middle Aged Neoplasms/genetics/pathology/*radiotherapy Radiation Tolerance/genetics/*physiology
Pubmed
Web of science
Create date
24/01/2008 17:16
Last modification date
20/08/2019 12:49
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