Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium.

Détails

ID Serval
serval:BIB_185FC1BBEB1E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium.
Périodique
Optics letters
Auteur(s)
Rappaz B., Charrière F., Depeursinge C., Magistretti P.J., Marquet P.
ISSN
0146-9592 (Print)
ISSN-L
0146-9592
Statut éditorial
Publié
Date de publication
01/04/2008
Peer-reviewed
Oui
Volume
33
Numéro
7
Pages
744-746
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Digital holographic microscopy (DHM) allows optical-path-difference (OPD) measurements with nanometric accuracy. OPD induced by transparent cells depends on both the refractive index (RI) of cells and their morphology. This Letter presents a dual-wavelength DHM that allows us to separately measure both the RI and the cellular thickness by exploiting an enhanced dispersion of the perfusion medium achieved by the utilization of an extracellular dye. The two wavelengths are chosen in the vicinity of the absorption peak of the dye, where the absorption is accompanied by a significant variation of the RI as a function of the wavelength.

Mots-clé
Absorption, Algorithms, Coloring Agents/pharmacology, Holography/methods, Image Interpretation, Computer-Assisted/methods, Microscopy, Phase-Contrast/methods, Models, Statistical, Optics and Photonics, Perfusion, Refractometry/methods, Reproducibility of Results, Schizosaccharomyces/metabolism, Signal Processing, Computer-Assisted
Pubmed
Web of science
Création de la notice
13/02/2009 14:15
Dernière modification de la notice
20/08/2019 12:48
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