Biased V beta usage in immature thymocytes is independent of DJ beta proximity and pT alpha pairing.

Détails

ID Serval
serval:BIB_18253
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Biased V beta usage in immature thymocytes is independent of DJ beta proximity and pT alpha pairing.
Périodique
Journal of Immunology
Auteur(s)
Wilson A., Maréchal C., MacDonald H.R.
ISSN
0022-1767
Statut éditorial
Publié
Date de publication
2001
Peer-reviewed
Oui
Volume
166
Numéro
1
Pages
51-57
Langue
anglais
Résumé
During thymus development, the TCR beta locus rearranges before the TCR alpha locus. Pairing of productively rearranged TCR beta-chains with an invariant pT alpha chain leads to the formation of a pre-TCR and subsequent expansion of immature pre-T cells. Essentially nothing is known about the TCR V beta repertoire in pre-T cells before or after the expression of a pre-TCR. Using intracellular staining, we show here that the TCR V beta repertoire is significantly biased at the earliest developmental stage in which VDJ beta rearrangement has occurred. Moreover (and in contrast to the V(H) repertoire in immature B cells), V beta repertoire biases in immature T cells do not reflect proximity of V beta gene segments to the DJ beta cluster, nor do they depend upon preferential V beta pairing with the pT alpha chain. We conclude that V gene repertoires in developing T and B cells are controlled by partially distinct mechanisms.
Mots-clé
Animals, Cell Differentiation/genetics, Cell Differentiation/immunology, Cell Division/genetics, Cell Division/immunology, Female, Flow Cytometry, Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor, Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, Intracellular Fluid/immunology, Intracellular Fluid/metabolism, Membrane Glycoproteins/biosynthesis, Membrane Glycoproteins/genetics, Mice, Mice, Inbred C57BL, Mice, Knockout, Multigene Family/genetics, Multigene Family/immunology, Protein Precursors/biosynthesis, Protein Precursors/genetics, Receptors, Antigen, T-Cell, alpha-beta/analysis, Receptors, Antigen, T-Cell, alpha-beta/biosynthesis, Staining and Labeling, T-Lymphocytes/chemistry, T-Lymphocytes/cytology, Thymus Gland/chemistry, Thymus Gland/cytology
Pubmed
Web of science
Création de la notice
19/11/2007 12:12
Dernière modification de la notice
20/08/2019 12:48
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