In vivo neurochemical measurements in cerebral tissues using a droplet-based monitoring system.

Détails

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Etat: Serval
Version: Final published version
ID Serval
serval:BIB_1793CE3D7607
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
In vivo neurochemical measurements in cerebral tissues using a droplet-based monitoring system.
Périodique
Nature communications
Auteur(s)
Petit-Pierre G., Colin P., Laurer E., Déglon J., Bertsch A., Thomas A., Schneider B.L., Renaud P.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Statut éditorial
Publié
Date de publication
01/11/2017
Peer-reviewed
Oui
Volume
8
Numéro
1
Pages
1239
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Direct collection of extracellular fluid (ECF) plays a central role in the monitoring of neurological disorders. Current approaches using microdialysis catheters are however drastically limited in term of temporal resolution. Here we show a functional in vivo validation of a droplet collection system included at the tip of a neural probe. The system comprises an advanced droplet formation mechanism which enables the collection of neurochemicals present in the brain ECF at high-temporal resolution. The probe was implanted in a rat brain and could successfully collect fluid samples organized in a train of droplets. A microfabricated target plate compatible with most of the surface-based detection methods was specifically developed for sample analysis. The time-resolved brain-fluid samples are analyzed using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The results provide a time evolution picture of the cerebral tissues neurochemical composition for selected elements known for their involvement in neurodegenerative diseases.
Mots-clé
Animals, Brain, Brain Chemistry, Calcium/analysis, Cerebrum/chemistry, Extracellular Fluid/chemistry, Female, Magnesium/analysis, Mass Spectrometry, Mercury/analysis, Microdialysis/methods, Potassium/analysis, Rats, Rats, Sprague-Dawley, Sodium/analysis, Time Factors
Pubmed
Web of science
Open Access
Oui
Création de la notice
03/11/2017 13:17
Dernière modification de la notice
08/05/2019 15:02
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