Bactericidal activity and mechanism of action of copper-sputtered flexible surfaces against multidrug-resistant pathogens.

Détails

ID Serval
serval:BIB_1792D7B2DBF4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Bactericidal activity and mechanism of action of copper-sputtered flexible surfaces against multidrug-resistant pathogens.
Périodique
Applied Microbiology and Biotechnology
Auteur(s)
Ballo M.K., Rtimi S., Mancini S., Kiwi J., Pulgarin C., Entenza J.M., Bizzini A.
ISSN
1432-0614 (Electronic)
ISSN-L
0175-7598
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
100
Numéro
13
Pages
5945-5953
Langue
anglais
Résumé
Using direct current magnetron sputtering (DCMS), we generated flexible copper polyester surfaces (Cu-PES) and investigated their antimicrobial activity against a range of multidrug-resistant (MDR) pathogens including eight Gram-positive isolates (three methicillin-resistant Staphylococcus aureus [MRSA], four vancomycin-resistant enterococci, one methicillin-resistant Staphylococcus epidermidis) and four Gram-negative strains (one extended-spectrum β-lactamase-producing [ESBL] Escherichia coli, one ESBL Klebsiella pneumoniae, one imipenem-resistant Pseudomonas aeruginosa, and one ciprofloxacin-resistant Acinetobacter baumannii). Bactericidal activity (≥3 log10 CFU reduction of the starting inoculum) was reached within 15-30 min exposure to Cu-PES. Antimicrobial activity of Cu-PES persisted in the absence of oxygen and against both Gram-positive and Gram-negative bacteria containing elevated levels of catalases, indicating that reactive oxygen species (ROS) do not play a primary role in the killing process. The decrease in cell viability of MRSA ATCC 43300 and Enterococcus faecalis V583 correlated with the progressive loss of cytoplasmic membrane integrity both under aerobic and anaerobic conditions, suggesting that Cu-PES mediated killing is primarily induced by disruption of the cytoplasmic membrane function. Overall, we here present novel antimicrobial copper surfaces with improved stability and sustainability and provide further insights into their mechanism of killing.

Mots-clé
Anti-Bacterial Agents/chemistry, Anti-Bacterial Agents/pharmacology, Bacterial Infections/microbiology, Copper/chemistry, Copper/pharmacology, Drug Evaluation, Preclinical, Drug Resistance, Bacterial, Gram-Negative Bacteria/drug effects, Gram-Positive Bacteria/drug effects, Humans, Microbial Sensitivity Tests, Nanoparticles/chemistry
Pubmed
Web of science
Création de la notice
16/08/2016 13:03
Dernière modification de la notice
20/08/2019 13:47
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