m<sup>6</sup>A modulates neuronal functions and sex determination in Drosophila.

Détails

ID Serval
serval:BIB_175BF2D91E8C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
m<sup>6</sup>A modulates neuronal functions and sex determination in Drosophila.
Périodique
Nature
Auteur(s)
Lence T., Akhtar J., Bayer M., Schmid K., Spindler L., Ho C.H., Kreim N., Andrade-Navarro M.A., Poeck B., Helm M., Roignant J.Y.
ISSN
1476-4687 (Electronic)
ISSN-L
0028-0836
Statut éditorial
Publié
Date de publication
08/12/2016
Peer-reviewed
Oui
Volume
540
Numéro
7632
Pages
242-247
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
N <sup>6</sup> -methyladenosine RNA (m <sup>6</sup> A) is a prevalent messenger RNA modification in vertebrates. Although its functions in the regulation of post-transcriptional gene expression are beginning to be unveiled, the precise roles of m <sup>6</sup> A during development of complex organisms remain unclear. Here we carry out a comprehensive molecular and physiological characterization of the individual components of the methyltransferase complex, as well as of the YTH domain-containing nuclear reader protein in Drosophila melanogaster. We identify the member of the split ends protein family, Spenito, as a novel bona fide subunit of the methyltransferase complex. We further demonstrate important roles of this complex in neuronal functions and sex determination, and implicate the nuclear YT521-B protein as a main m <sup>6</sup> A effector in these processes. Altogether, our work substantially extends our knowledge of m <sup>6</sup> A biology, demonstrating the crucial functions of this modification in fundamental processes within the context of the whole animal.
Mots-clé
Adenosine/analogs & derivatives, Adenosine/metabolism, Alternative Splicing, Animals, Behavior, Animal/physiology, Drosophila Proteins/chemistry, Drosophila Proteins/deficiency, Drosophila Proteins/genetics, Drosophila Proteins/metabolism, Drosophila melanogaster/cytology, Drosophila melanogaster/enzymology, Drosophila melanogaster/physiology, Female, Male, Methyltransferases/chemistry, Methyltransferases/metabolism, Nervous System/metabolism, Neurons/physiology, Nuclear Proteins/chemistry, Nuclear Proteins/deficiency, Nuclear Proteins/genetics, Nuclear Proteins/metabolism, Phenotype, Protein Subunits/chemistry, Protein Subunits/metabolism, RNA-Binding Proteins/genetics, Sex Determination Processes/genetics, Sex Determination Processes/physiology
Pubmed
Web of science
Création de la notice
28/10/2019 12:54
Dernière modification de la notice
29/10/2019 6:26
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