Article: article from journal or magazin.
Yeast Ran-binding protein 1 (Yrb1) shuttles between the nucleus and cytoplasm and is exported from the nucleus via a CRM1 (XPO1)-dependent pathway.
Molecular and Cellular Biology
Publication types: Journal Article
The RanGTP-binding protein RanBP1, which is located in the cytoplasm, has been implicated in release of nuclear export complexes from the cytoplasmic side of the nuclear pore complex. Here we show that Yrb1 (the yeast homolog of RanBP1) shuttles between the nucleus and the cytoplasm. Nuclear import of Yrb1 is a facilitated process that requires a short basic sequence within the Ran-binding domain (RBD). By contrast, nuclear export of Yrb1 requires an intact RBD, which forms a ternary complex with the Xpo1 (Crm1) NES receptor in the presence of RanGTP. Nuclear export of Yrb1, however, is insensitive towards leptomycin B, suggesting a novel type of substrate recognition between Yrb1 and Xpo1. Taken together, these data suggest that ongoing nuclear import and export is an important feature of Yrb1 function in vivo.
Animals, Biological Transport, Carrier Proteins/metabolism, Cell Nucleus/metabolism, Cytoplasm/metabolism, Fungal Proteins/metabolism, Karyopherins, Mice, Nuclear Proteins/metabolism, Receptors, Cytoplasmic and Nuclear, Saccharomyces cerevisiae/metabolism
Web of science
Last modification date