Behavior of endogenous tumor-associated macrophages assessed in vivo using a functionalized nanoparticle.

Détails

ID Serval
serval:BIB_17196E58EB3F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Behavior of endogenous tumor-associated macrophages assessed in vivo using a functionalized nanoparticle.
Périodique
Neoplasia
Auteur(s)
Leimgruber A., Berger C., Cortez-Retamozo V., Etzrodt M., Newton A.P., Waterman P., Figueiredo J.L., Kohler R.H., Elpek N., Mempel T.R., Swirski F.K., Nahrendorf M., Weissleder R., Pittet M.J.
ISSN
1476-5586[electronic]
Statut éditorial
Publié
Date de publication
2009
Volume
11
Numéro
5
Pages
459-468, 2 p following 468
Langue
anglais
Résumé
Tumor-associated macrophages (TAMs) invade the tumor stroma in many cancers, yet their role is incompletely understood. To visualize and better understand these critical cells in tumor progression, we screened a portfolio of rationally selected, injectable agents to image endogenous TAMs ubiquitously in three different cancer models (colon carcinoma, lung adenocarcinoma, and soft tissue sarcoma). AMTA680, a functionally derivatized magneto-fluorescent nanoparticle, labeled a subset of myeloid cells with an "M2" macrophage phenotype, whereas other neighboring cells, including tumor cells and a variety of other leukocytes, remained unlabeled. We further show that AMTA680-labeled endogenous TAMs are not altered and can be tracked noninvasively at different resolutions and using various imaging modalities, e.g., fluorescence molecular tomography, magnetic resonance imaging, and multiphoton and confocal intravital microscopy. Quantitative assessment of TAM distribution and activity in vivo identified that these cells cluster in delimited foci within tumors, show relatively low motility, and extend cytoplasmic protrusions for prolonged physical interactions with neighboring tumor cells. Noninvasive imaging can also be used to monitor TAM-depleting regimen quantitatively. Thus, AMTA680 or related cell-targeting agents represent appropriate injectable vehicles for in vivo analysis of the tumor microenvironment.
Mots-clé
Animals, Diagnostic Imaging/methods, Immunohistochemistry, Macrophages/cytology, Magnetic Resonance Imaging, Metal Nanoparticles/diagnostic use, Mice, Microscopy, Confocal, Neoplasms/immunology, Reverse Transcriptase Polymerase Chain Reaction
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/06/2009 16:35
Dernière modification de la notice
08/05/2019 15:00
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