Cx36 Is a Target of Beta2/NeuroD1, Which Associates with Prenatal Differentiation of Insulin-producing β Cells.

Détails

ID Serval
serval:BIB_16DFDB396AFB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cx36 Is a Target of Beta2/NeuroD1, Which Associates with Prenatal Differentiation of Insulin-producing β Cells.
Périodique
Journal of Membrane Biology
Auteur(s)
Nlend R.N., Aït-Lounis A., Allagnat F., Cigliola V., Charollais A., Reith W., Haefliger J.A., Meda P.
ISSN
1432-1424 (Electronic)
ISSN-L
0022-2631
Statut éditorial
Publié
Date de publication
2012
Peer-reviewed
Oui
Volume
245
Numéro
5-6
Pages
263-273
Langue
anglais
Notes
Publication types: Journal Article
Résumé
The insulin-producing β cells of pancreatic islets are coupled by connexin36 (Cx36) channels. To investigate what controls the expression of this connexin, we have investigated its pattern during mouse pancreas development, and the influence of three transcription factors that are critical for β-cell development and differentiation. We show that (1) the Cx36 gene (Gjd2) is activated early in pancreas development and is markedly induced at the time of the surge of the transcription factors that determine β-cell differentiation; (2) the cognate protein is detected about a week later and is selectively expressed by β cells throughout the prenatal development of mouse pancreas; (3) a 2-kbp fragment of the Gjd2 promoter, which contains three E boxes for the binding of the bHLH factor Beta2/NeuroD1, ensures the expression of Cx36 by β cells; and (4) Beta2/NeuroD1 binds to these E boxes and, in the presence of the E47 ubiquitous cofactor, transactivates the Gjd2 promoter. The data identify Cx36 as a novel early marker of β cells and as a target of Beta2/NeuroD1, which is essential for β-cell development and differentiation.
Pubmed
Web of science
Création de la notice
27/08/2012 18:38
Dernière modification de la notice
20/08/2019 13:46
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