Article: article from journal or magazin.
EDS5, an essential component of salicylic acid-dependent signaling for disease resistance in Arabidopsis, is a member of the MATE transporter family.
The eds5 mutant of Arabidopsis (earlier named sid1) was shown previously to accumulate very little salicylic acid and PR-1 transcript after pathogen inoculation and to be hypersusceptible to pathogens. We have isolated EDS5 by positional cloning and show that it encodes a protein with a predicted series of nine to 11 membrane-spanning domains and a coil domain at the N terminus. EDS5 is homologous with members of the MATE (multidrug and toxin extrusion) transporter family. EDS5 expression is very low in unstressed plants and strongly induced by pathogens and UV-C light. The transcript starts to accumulate 2 hr after inoculation of Arabidopsis with an avirulent strain of Pseudomonas syringae or UV-C light exposure, and it stays induced for approximately 2 days. EDS5 also is expressed after treatments with salicylic acid, indicating a possible positive feedback regulation. EDS5 expression after infection by certain pathogens as well as after UV-C light exposure depends on the pathogen response proteins EDS1, PAD4, and NDR1, indicating that the signal transduction pathways after UV-C light exposure and pathogen inoculation share common elements.
Amino Acid Sequence, Arabidopsis/drug effects, Arabidopsis/genetics, Arabidopsis Proteins/genetics, Arabidopsis Proteins/radiation effects, Base Sequence, Biological Transport, Carboxylic Ester Hydrolases/genetics, Chromosome Mapping, Cloning, Molecular, DNA, Complementary/chemistry, DNA, Complementary/genetics, DNA-Binding Proteins/genetics, Gene Expression Regulation, Plant/drug effects, Gene Expression Regulation, Plant/radiation effects, Immunity, Innate/genetics, Membrane Proteins/genetics, Membrane Transport Proteins/genetics, Membrane Transport Proteins/radiation effects, Molecular Sequence Data, Mutation, Plant Diseases/genetics, Plant Diseases/microbiology, Pseudomonas/growth & development, Salicylic Acid/pharmacology, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Signal Transduction/drug effects, Signal Transduction/radiation effects, Transcription Factors/genetics, Ultraviolet Rays
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