Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards.
Details
Serval ID
serval:BIB_162A26579E44
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards.
Journal
Nature communications
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
03/10/2024
Peer-reviewed
Oui
Volume
15
Number
1
Pages
8562
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Abstract
In this community effort, we compare measurements between 34 laboratories from 19 countries, utilizing mixtures of labelled authentic synthetic standards, to quantify by mass spectrometry four clinically used ceramide species in the NIST (National Institute of Standards and Technology) human blood plasma Standard Reference Material (SRM) 1950, as well as a set of candidate plasma reference materials (RM 8231). Participants either utilized a provided validated method and/or their method of choice. Mean concentration values, and intra- and inter-laboratory coefficients of variation (CV) were calculated using single-point and multi-point calibrations, respectively. These results are the most precise (intra-laboratory CVs ≤ 4.2%) and concordant (inter-laboratory CVs < 14%) community-derived absolute concentration values reported to date for four clinically used ceramides in the commonly analyzed SRM 1950. We demonstrate that calibration using authentic labelled standards dramatically reduces data variability. Furthermore, we show how the use of shared RM can correct systematic quantitative biases and help in harmonizing lipidomics. Collectively, the results from the present study provide a significant knowledge base for translation of lipidomic technologies to future clinical applications that might require the determination of reference intervals (RIs) in various human populations or might need to estimate reference change values (RCV), when analytical variability is a key factor for recall during multiple testing of individuals.
Keywords
Humans, Ceramides/blood, Reference Standards, Calibration, Laboratories/standards, Mass Spectrometry/methods, Lipidomics/methods, Reproducibility of Results
Pubmed
Open Access
Yes
Create date
11/10/2024 12:24
Last modification date
11/10/2024 19:15