Differential GAP requirement for Cdc42-GTP polarization during proliferation and sexual reproduction.

Détails

Ressource 1Télécharger: jcb.201806016.full.pdf (2869.03 [Ko])
Etat: Public
Version: Final published version
Licence: Non spécifiée
ID Serval
serval:BIB_15FB6294D95C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Differential GAP requirement for Cdc42-GTP polarization during proliferation and sexual reproduction.
Périodique
The Journal of cell biology
Auteur(s)
Gallo Castro D., Martin S.G.
ISSN
1540-8140 (Electronic)
ISSN-L
0021-9525
Statut éditorial
Publié
Date de publication
03/12/2018
Peer-reviewed
Oui
Volume
217
Numéro
12
Pages
4215-4229
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The formation of a local zone of Cdc42 GTPase activity, which governs cell polarization in many cell types, requires not only local activation but also switch-off mechanisms. In this study, we identify Rga3, a paralog of Rga4, as a novel Cdc42 GTPase-activating protein (GAP) in the fission yeast Schizosaccharomyces pombe Contrary to Rga4, Rga3 localizes with Cdc42-GTP to sites of polarity. Rga3 is dispensable for cell polarization during mitotic growth, but it limits the lifetime of unstable Cdc42-GTP patches that underlie cell pairing during sexual reproduction, masking a partly compensatory patch-wandering motion. In consequence, cells lacking rga3 hyperpolarize and lose out in mating competition. Rga3 synergizes with the Cdc42 GAPs Rga4 and Rga6 to restrict Cdc42-GTP zone sizes during mitotic growth. Surprisingly, triple-mutant cells, which are almost fully round, retain pheromone-dependent dynamic polarization of Cdc42-GTP, extend a polarized projection, and mate. Thus, the requirement for Cdc42-GTP hydrolysis by GAPs is distinct during polarization by intrinsic or extrinsic cues.
Mots-clé
GTPase-Activating Proteins/genetics, GTPase-Activating Proteins/metabolism, Guanosine Triphosphate/genetics, Guanosine Triphosphate/metabolism, Mitosis/physiology, Schizosaccharomyces/enzymology, Schizosaccharomyces/genetics, Schizosaccharomyces pombe Proteins/genetics, Schizosaccharomyces pombe Proteins/metabolism, cdc42 GTP-Binding Protein/genetics, cdc42 GTP-Binding Protein/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
16/10/2018 11:07
Dernière modification de la notice
28/09/2019 5:08
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