Detecting Release of Bacterial dsDNA into the Host Cytosol Using Fluorescence Microscopy.

Détails

ID Serval
serval:BIB_14D54D75F8C7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Detecting Release of Bacterial dsDNA into the Host Cytosol Using Fluorescence Microscopy.
Périodique
Methods in molecular biology
Auteur(s)
Dreier R.F., Santos J.C., Broz P.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
1714
Pages
199-213
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Recognition of pathogens by the innate immune system relies on germline-encoded pattern recognition receptors (PRRs) that recognize unique microbial molecules, so-called pathogen-associated molecular patterns (PAMPs). Nucleic acids and their derivatives are one of the most important groups of PAMPs, and are recognized by a number of surface-associated as well as cytosolic PRRs. Cyclic GMP-AMP synthase (cGAS) recognizes the presence of pathogen- or host-derived dsDNA in the cytosol and initiates type-I-IFN production. Here, we describe a methodology that allows for evaluating the association of cGAS with released bacterial dsDNA during Francisella novicida infection of macrophages, by fluorescence confocal microscopy. This method can be adapted to the study of cGAS-dependent responses elicited by other intracellular bacterial pathogens and in other cell types.

Mots-clé
F. novicida, Fluorescence confocal microscopy, Innate immunity, cGAS, dsDNA
Pubmed
Création de la notice
30/11/2017 11:37
Dernière modification de la notice
21/08/2019 6:36
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