The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand.

Détails

Ressource 1Télécharger: journal.pone.0165850.PDF (2969.64 [Ko])
Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_14197457242A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The Genome of the Toluene-Degrading Pseudomonas veronii Strain 1YdBTEX2 and Its Differential Gene Expression in Contaminated Sand.
Périodique
PloS one
Auteur(s)
Morales M., Sentchilo V., Bertelli C., Komljenovic A., Kryuchkova-Mostacci N., Bourdilloud A., Linke B., Goesmann A., Harshman K., Segers F., Delapierre F., Fiorucci D., Seppey M., Trofimenco E., Berra P., El Taher A., Loiseau C., Roggero D., Sulfiotti M., Etienne A., Ruiz Buendia G., Pillard L., Escoriza A., Moritz R., Schneider C., Alfonso E., Ben Jeddou F., Selmoni O., Resch G., Greub G., Emery O., Dubey M., Pillonel T., Robinson-Rechavi M., van der Meer J.R.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
11
Numéro
11
Pages
e0165850
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish

Résumé
The natural restoration of soils polluted by aromatic hydrocarbons such as benzene, toluene, ethylbenzene and m- and p-xylene (BTEX) may be accelerated by inoculation of specific biodegraders (bioaugmentation). Bioaugmentation mainly involves introducing bacteria that deploy their metabolic properties and adaptation potential to survive and propagate in the contaminated environment by degrading the pollutant. In order to better understand the adaptive response of cells during a transition to contaminated material, we analyzed here the genome and short-term (1 h) changes in genome-wide gene expression of the BTEX-degrading bacterium Pseudomonas veronii 1YdBTEX2 in non-sterile soil and liquid medium, both in presence or absence of toluene. We obtained a gapless genome sequence of P. veronii 1YdBTEX2 covering three individual replicons with a total size of 8 Mb, two of which are largely unrelated to current known bacterial replicons. One-hour exposure to toluene, both in soil and liquid, triggered massive transcription (up to 208-fold induction) of multiple gene clusters, such as toluene degradation pathway(s), chemotaxis and toluene efflux pumps. This clearly underlines their key role in the adaptive response to toluene. In comparison to liquid medium, cells in soil drastically changed expression of genes involved in membrane functioning (e.g., lipid composition, lipid metabolism, cell fatty acid synthesis), osmotic stress response (e.g., polyamine or trehalose synthesis, uptake of potassium) and putrescine metabolism, highlighting the immediate response mechanisms of P. veronii 1YdBTEX2 for successful establishment in polluted soil.

Mots-clé
Dehydration, Gene Expression Profiling, Microarray Analysis, Micrococcaceae/genetics, Micrococcaceae/metabolism, Pseudomonas/genetics, Pseudomonas/growth & development, Pseudomonas/metabolism, Sphingomonas/genetics, Sphingomonas/metabolism, Water Pollutants/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/11/2016 12:27
Dernière modification de la notice
08/05/2019 14:48
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