Reduced peroxisomal citrate synthase activity increases substrate availability for polyhydroxyalkanoate biosynthesis in plant peroxisomes.

Détails

ID Serval
serval:BIB_137DC9FB61B0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Reduced peroxisomal citrate synthase activity increases substrate availability for polyhydroxyalkanoate biosynthesis in plant peroxisomes.
Périodique
Plant Biotechnology Journal
Auteur(s)
Tilbrook K., Poirier Y., Gebbie L., Schenk P.M., McQualter R.B., Brumbley S.M.
ISSN
1467-7652 (Electronic)
ISSN-L
1467-7644
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
12
Numéro
8
Pages
1044-1052
Langue
anglais
Résumé
Polyhydroxyalkanoates (PHAs) are bacterial carbon storage polymers used as renewable, biodegradable plastics. PHA production in plants may be a way to reduce industrial PHA production costs. We recently demonstrated a promising level of peroxisomal PHA production in the high biomass crop species sugarcane. However, further production strategies are needed to boost PHA accumulation closer to commercial targets. Through exogenous fatty acid feeding of Arabidopsis thaliana plants that contain peroxisome-targeted PhaA, PhaB and PhaC enzymes from Cupriavidus necator, we show here that the availability of substrates derived from the β-oxidation cycle limits peroxisomal polyhydroxybutyrate (PHB) biosynthesis. Knockdown of peroxisomal citrate synthase activity using artificial microRNA increased PHB production levels approximately threefold. This work demonstrates that reduction of peroxisomal citrate synthase activity may be a valid metabolic engineering strategy for increasing PHA production in other plant species.
Mots-clé
polyhydroxyalkanoate, citrate synthase, beta-oxidation, peroxisome, plant metabolic engineering, Arabidopsis
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/10/2014 11:50
Dernière modification de la notice
20/08/2019 12:42
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