Mapping of HLA epitopes recognized by H-2-restricted cytotoxic T lymphocytes specific for HLA using recombinant genes and synthetic peptides

Details

Serval ID
serval:BIB_12A64CAF7C0E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Mapping of HLA epitopes recognized by H-2-restricted cytotoxic T lymphocytes specific for HLA using recombinant genes and synthetic peptides
Journal
Journal of Immunology
Author(s)
Pala  P., Corradin  G., Strachan  T., Sodoyer  R., Jordan  B. R., Cerottini  J. C., Maryanski  J. L.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
02/1988
Volume
140
Number
3
Pages
871-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb 1
Abstract
Immunization of DBA/2 (H-2d) mice with syngeneic P815 tumor cell transfectants that express HLA class I genes elicits CTL that recognize HLA in the context of H-2Kd molecules. Anti-HLA-CW3 CTL cross-react to a variable extent on the related alleles A3 and A24. Using a panel of target cells expressing native or recombinant HLA genes, we could map the epitope recognized by a CTL clone specific for CW3 to the second external (alpha 2) domain of CW3. Moreover, the epitope recognized by this clone could be mimicked by incubating P815 (HLA negative) target cells with a synthetic peptide corresponding to the C-terminal 12 amino acids of the CW3 alpha 2 domain (residues 171 to 182). Other independent anti-CW3 CTL clones with different fine specificities recognized the same CW3 peptide. In contrast, CTL clones specific for HLA-A24 or HLA-A3 that did not lyse P815-CW3 transfectants did not recognize this peptide. The CW3 peptide could be recognized on other tumor cell targets that were also of H-2d origin, but not on those of H-2b or H-2k origin. The requirement for the expression of H-2Kd by the target cells was directly demonstrated using L cell Kd transfectants. Our results suggest that the CTL response of DBA/2 mice immunized with P815-CW3 transfectants is predominantly Kd restricted and focused on epitopes contained within the 12 C-terminal amino acids of the alpha 2 domain.
Keywords
Animals Cross Reactions Cytotoxicity Tests, Immunologic *DNA, Recombinant/immunology Epitopes/analysis/*genetics/immunology H-2 Antigens/*genetics HLA Antigens/analysis/*genetics/immunology *HLA-C Antigens Humans Lymphocyte Activation Mice Mice, Inbred DBA Peptides/*genetics/immunology/pharmacology T-Lymphocytes, Cytotoxic/*immunology Transfection
Pubmed
Web of science
Create date
28/01/2008 11:14
Last modification date
20/08/2019 12:40
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