Microheterogeneity of serum glycoproteins in patients with chronic alcohol abuse compared with carbohydrate-deficient glycoprotein syndrome type I.

Details

Serval ID
serval:BIB_12784
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Microheterogeneity of serum glycoproteins in patients with chronic alcohol abuse compared with carbohydrate-deficient glycoprotein syndrome type I.
Journal
Clinical Chemistry
Author(s)
Henry H., Froehlich F., Perret R., Tissot J.D., Eilers-Messerli B., Lavanchy D., Dionisi-Vici C., Gonvers J.J., Bachmann C.
ISSN
0009-9147
Publication state
Published
Issued date
1999
Peer-reviewed
Oui
Volume
45
Number
9
Pages
1408-1413
Language
english
Abstract
BACKGROUND: Chronic alcohol abuse alters the normal N-glycosylation of transferrin, producing the carbohydrate-deficient transferrin isoforms. This alteration could be similar to that present in patients with carbohydrate-deficient glycoprotein syndrome type 1 (CDG1). We thus compared the alterations of N-glycans present in patients with alcoholism and patients with CDG1. METHODS: The N-glycans of serum glycoproteins were compared in sera of patients with alcoholism, patients with CDG1, and controls by two-dimensional electrophoresis, neuraminidase, peptide:N-glycosidase F, and endoglycosidase F2 treatments. A specific antibody directed against the amino acid sequence surrounding the N-432 N-glycosylation site of transferrin was prepared (SZ-350 antibody). RESULTS: In patients with alcoholism, the abnormal transferrin and alpha(1)-antitrypsin isoforms were devoid of a variable number of entire N-glycan moieties and were identical with those present in CDG1. In the serum of patients with alcoholism, this finding was less pronounced than in CDG1. In contrast to CDG1, there was no decrease in clusterin or serum amyloid P in patients with alcoholism. The SZ-350 antibody recognized only transferrin isoforms with one or no N-glycan moieties. CONCLUSION: Antibodies directed against specific N-glycosylation sites of glycoproteins could be useful for developing more specific immunochemical tests for the diagnosis of chronic alcohol abuse.
Keywords
Alcoholism/blood, Amidohydrolases, Antibody Specificity, Blood Proteins/analysis, Blood Proteins/chemistry, Carbohydrate-Deficient Glycoprotein Syndrome/blood, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Glycoproteins/blood, Glycoproteins/chemistry, Glycosylation, Humans, Immunoblotting, Neuraminidase, Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase, Polysaccharides/metabolism, Transferrin/chemistry, Transferrin/immunology
Pubmed
Web of science
Create date
19/11/2007 12:04
Last modification date
20/08/2019 12:40
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