Global transcriptome sequencing identifies chlamydospore specific markers in Candida albicans and Candida dubliniensis.

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Serval ID
serval:BIB_1253E5DD657F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Global transcriptome sequencing identifies chlamydospore specific markers in Candida albicans and Candida dubliniensis.
Journal
PLoS One
Author(s)
Palige K., Linde J., Martin R., Böttcher B., Citiulo F., Sullivan D.J., Weber J., Staib C., Rupp S., Hube B., Morschhäuser J., Staib P.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2013
Volume
8
Number
4
Pages
e61940
Language
english
Abstract
Candida albicans and Candida dubliniensis are pathogenic fungi that are highly related but differ in virulence and in some phenotypic traits. During in vitro growth on certain nutrient-poor media, C. albicans and C. dubliniensis are the only yeast species which are able to produce chlamydospores, large thick-walled cells of unknown function. Interestingly, only C. dubliniensis forms pseudohyphae with abundant chlamydospores when grown on Staib medium, while C. albicans grows exclusively as a budding yeast. In order to further our understanding of chlamydospore development and assembly, we compared the global transcriptional profile of both species during growth in liquid Staib medium by RNA sequencing. We also included a C. albicans mutant in our study which lacks the morphogenetic transcriptional repressor Nrg1. This strain, which is characterized by its constitutive pseudohyphal growth, specifically produces masses of chlamydospores in Staib medium, similar to C. dubliniensis. This comparative approach identified a set of putatively chlamydospore-related genes. Two of the homologous C. albicans and C. dubliniensis genes (CSP1 and CSP2) which were most strongly upregulated during chlamydospore development were analysed in more detail. By use of the green fluorescent protein as a reporter, the encoded putative cell wall related proteins were found to exclusively localize to C. albicans and C. dubliniensis chlamydospores. Our findings uncover the first chlamydospore specific markers in Candida species and provide novel insights in the complex morphogenetic development of these important fungal pathogens.
Pubmed
Web of science
Open Access
Yes
Create date
16/05/2013 16:48
Last modification date
20/08/2019 12:40
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