A TBP complex essential for transcription from TATA-less but not TATA-containing RNA polymerase III promoters is part of the TFIIIB fraction.

Détails

ID Serval
serval:BIB_117E94AC59A4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A TBP complex essential for transcription from TATA-less but not TATA-containing RNA polymerase III promoters is part of the TFIIIB fraction.
Périodique
Cell
Auteur(s)
Lobo S.M., Tanaka M., Sullivan M.L., Hernandez N.
ISSN
0092-8674
Statut éditorial
Publié
Date de publication
12/1992
Peer-reviewed
Oui
Volume
71
Numéro
6
Pages
1029-1040
Langue
anglais
Résumé
The TATA box-binding protein TBP directs transcription by all three eukaryotic RNA polymerases. In mammalian cells, TBP is found in at least three different complexes: SL1, D-TFIID, and B-TFIID. While SL1 and D-TFIID are involved in RNA polymerase I and II transcription, respectively, no unique function has been assigned to the B-TFIID complex. Here we show that the TFIIIB fraction required for RNA polymerase III transcription contains two separable components, one of which is a TBP-containing complex that may correspond to B-TFIID. For transcription of TATA-less RNA polymerase III genes such as the VAI, 5S, and 7SL genes, this complex cannot be replaced by either TBP alone or the D-TFIID complex. In contrast, TBP alone is active for basal transcription from the TATA-containing U6 promoter. This indicates different requirements for recruiting TBP to TATA-less and TATA-containing RNA polymerase III promoters.
Mots-clé
Base Sequence, DNA Polymerase III, DNA-Binding Proteins, DNA-Directed RNA Polymerases, Molecular Sequence Data, Multienzyme Complexes, Promoter Regions, Genetic, TATA-Box Binding Protein, Transcription Factor TFIIIB, Transcription Factors, Transcription, Genetic
Pubmed
Web of science
Création de la notice
21/01/2008 17:34
Dernière modification de la notice
20/08/2019 13:39
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