Article: article from journal or magazin.
A novel member of the interferon receptor family complements functionality of the murine interferon gamma receptor in human cells.
Expression of the human interferon gamma receptor (IFN-gamma R) in mouse cells is not sufficient to confer biological responsiveness to human IFN-gamma and vice versa. An additional species-specific component is required for signal transduction. We identified this cofactor by expression cloning in simian COS cells stably transfected with the nonfunctional murine IFN-gamma R and a IFN-gamma-inducible reporter construct encoding the human Tac antigen (interleukin-2 receptor alpha chain, CD25). A cDNA clone was obtained that, upon stable transfection, rendered human HEp-2 cells expressing the murine IFN-gamma R fully responsive to murine IFN-gamma. This cDNA encodes a novel 332 amino acid type I transmembrane protein that belongs to the IFN receptor family and that we designate IFN-gamma R beta chain.
Amino Acid Sequence, Animals, Base Sequence, Chromosomes, Human, Pair 21, Cloning, Molecular, DNA, Complementary/genetics, Genes, Genetic Complementation Test, Humans, Mice, Molecular Sequence Data, Receptors, Interferon/chemistry, Receptors, Interferon/genetics, Sequence Alignment, Sequence Homology, Amino Acid
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