The collecting tubule of Amphiuma. I. Electrophysiological characterization
Details
Serval ID
serval:BIB_107D4433A4D4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The collecting tubule of Amphiuma. I. Electrophysiological characterization
Journal
American Journal of Physiology
ISSN
0002-9513 (Print)
Publication state
Published
Issued date
12/1987
Volume
253
Number
6 Pt 2
Pages
F1263-72
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Dec
Research Support, U.S. Gov't, P.H.S. --- Old month value: Dec
Abstract
Single collecting tubules of Amphiuma kidneys were perfused in vitro to characterize their electrophysiological properties. The lumen-negative potential (-24 mV) was abolished by amiloride in the lumen and by ouabain in the bath. Ion substitution experiments in the lumen demonstrated the presence of a large sodium conductance in the apical cell membrane, but no evidence was obtained for a significant potassium or chloride conductance. Ion substitutions in the bath solution and the depolarizing effect of barium on the basolateral membrane potential demonstrated the presence of a large potassium conductance in the basolateral cell membrane. Measurements of dilution potentials in amiloride-treated tubules revealed a modest cation selectivity of the paracellular pathway. These results support a cell model in which sodium reabsorption occurs by electrodiffusion across the apical cell membrane and active transport across the basolateral cell membrane. The absence of a detectable potassium conductance in the apical cell membrane suggests that secretion of this ion cannot take place by diffusion from cell to lumen.
Keywords
Algorithms
Amiloride/pharmacology
Animals
Barium/pharmacology
Electric Conductivity
Electrophysiology
Female
Kidney Tubules/*physiology
Kidney Tubules, Collecting/*physiology
Male
Ouabain/pharmacology
Reference Values
Urodela/*physiology
Pubmed
Web of science
Create date
24/01/2008 13:38
Last modification date
20/08/2019 13:37