Article: article from journal or magazin.
Murine autoimmune gastritis and the gastric H,K-ATPase: insights from a new model and autoantibody detection system
Verhandlungen der Deutschen Gesellschaft fur Pathologie
Human type A chronic gastritis or autoimmune gastritis (AIG) is associated with gastric H,K-ATPase-specific autoantibodies (HKAb). The pathogenic role of the HKAb and the triggering autoantigen(s) are unknown. In a mouse model, neonatal thymectomy (nTx) induces AIG, which is likely T cell mediated, although HKAb are always present. Our aim is to study the role of the H,K-ATPase in the initiation of AIG. The direct involvement of the H,K-ATPase in the onset of AIG is suggested by the following findings. AIG appears at the age of 1 month in susceptible BALB.D2 mice, i.e. the time at which H,K-ATPase expression reaches adult levels. A new HKAb assay system based on immunoprecipitation of native H,K-ATPase expressed in Xenopus oocytes has revealed that the early lesion is already associated with low titers of HKAb. Injection of gastric membranes, rich in H,K-ATPase, into neonatal BALB.D2 mice without adjuvant induces a persisting AIG. This new model for AIG will provide the means to identify which H,K-ATPase subunit triggers AIG.
Animals Animals, Newborn Autoimmune Diseases/enzymology/immunology/*pathology Gastritis/enzymology/immunology/*pathology H(+)-K(+)-Exchanging ATPase/biosynthesis/*immunology Humans Mice Mice, Inbred Strains T-Lymphocytes/immunology Thymectomy
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