Unexpectedly late expression of intracellular CD3epsilon and TCR gammadelta proteins during adult thymus development.

Détails

Ressource 1Télécharger: serval:BIB_10582.P001 (515.33 [Ko])
Etat: Public
Version: de l'auteur
Licence: Non spécifiée
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
ID Serval
serval:BIB_10582
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Unexpectedly late expression of intracellular CD3epsilon and TCR gammadelta proteins during adult thymus development.
Périodique
International Immunology
Auteur(s)
Wilson A., Capone M., MacDonald H.R.
ISSN
0953-8178
Statut éditorial
Publié
Date de publication
1999
Peer-reviewed
Oui
Volume
11
Numéro
10
Pages
1641-1650
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
During adult thymus development immature CD4(-)CD8(-) [double-negative (DN)] precursor cells pass through four phenotypically distinct stages defined by expression of CD44 and CD25: CD44(hi)CD25(-) (DN1), CD44(hi)CD25(+) (DN2), CD44(lo)CD25(+) (DN3) and CD44(lo)CD25(-) (DN4). Although it is well established that the TCR beta, gamma and delta genes are rearranged and expressed in association with the CD3 components in DN thymocytes, the precise timing of expression of the TCR and CD3 proteins has not been determined. In this report we have utilized a sensitive intracellular (ic) staining technique to analyze the expression of ic CD3epsilon, TCR beta and TCR gammadelta proteins in immature DN subsets. As expected from previous studies of TCR beta rearrangement and mRNA expression, icTCR beta(+) cells were first detected in the DN3 subset and their proportion increased thereafter. Surprisingly, however, both icCD3epsilon(+) and icTCR gammadelta(+) cells were detected at later stages of development than was predicted by molecular studies. In particular icCD3epsilon protein expression coincided with the transition from the DN2 to DN3 stage of development, whereas icTCR gammadelta protein expression was only detected in a minor subset of DN4 cells. The implications of these findings for alphabeta lineage divergence will be discussed.
Mots-clé
Age Factors, Animals, Antigens, CD3, Antigens, CD44/metabolism, Biological Markers, Cell Differentiation, Female, Flow Cytometry, Mice, Mice, Inbred C57BL, Mice, Knockout, Receptors, Antigen, T-Cell/metabolism, Receptors, Antigen, T-Cell, alpha-beta/deficiency, Receptors, Antigen, T-Cell, alpha-beta/metabolism, Receptors, Antigen, T-Cell, gamma-delta/deficiency, Receptors, Antigen, T-Cell, gamma-delta/metabolism, Receptors, Interleukin-2/metabolism, T-Lymphocytes/cytology, T-Lymphocytes/metabolism, Thymus Gland/growth &amp, development, Thymus Gland/metabolism, Time Factors
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/11/2007 12:00
Dernière modification de la notice
25/09/2019 6:08
Données d'usage