Article: article from journal or magazin.
Cutting edge: NKT cell development is selectively impaired in Fyn- deficient mice.
Journal of Immunology
Most NK1.1+ T (NKT) cells express a biased TCRalphabeta repertoire that is positively selected by the monomorphic MHC class I-like molecule CD1d. The development of CD1d-dependent NKT cells is thymus dependent but, in contrast to conventional T cells, requires positive selection by cells of hemopoietic origin. Here, we show that the Src protein tyrosine kinase Fyn is required for development of CD1d-dependent NKT cells but not for the development of conventional T cells. In contrast, another Src kinase, Lck, is required for the development of both NKT and T cells. Impaired NKT cell development in Fyn-deficient mice cannot be rescued by transgenic expression of CD8, which is believed to increase the avidity of CD1d recognition by NKT cells. Taken together, our data reveal a selective and nonredundant role for Fyn in NKT cell development.
Animals, Antigens/genetics, Antigens, CD4/biosynthesis, Antigens, Ly, Antigens, Surface, Cell Differentiation/genetics, Cell Differentiation/immunology, Killer Cells, Natural/immunology, Killer Cells, Natural/metabolism, Lectins, C-Type, Lymphocyte Activation/genetics, Lymphocyte Count, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, NK Cell Lectin-Like Receptor Subfamily B, Organ Specificity/genetics, Organ Specificity/immunology, Proteins/genetics, Proto-Oncogene Proteins/deficiency, Proto-Oncogene Proteins/genetics, Proto-Oncogene Proteins c-fyn, Receptors, Antigen, T-Cell/biosynthesis, Receptors, Antigen, T-Cell, alpha-beta/genetics, Receptors, Antigen, T-Cell, alpha-beta/physiology, Signal Transduction/immunology, T-Lymphocyte Subsets/immunology, T-Lymphocyte Subsets/metabolism
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