Article: article from journal or magazin.
Absolute requirement for the pre-T cell receptor alpha chain during NK1.1+ TCRalphabeta cell development.
European Journal of Immunology
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Most natural killer T (NKT) cells express a highly skewed alphabeta TCR repertoire, consisting of an invariant V alpha14-J alpha281 chain paired preferentially with a polyclonal Vbeta8.2 chain. This repertoire is positively selected by the monomorphic CD1d molecule expressed on cells of hematopoietic origin. The origin of NKT cells and their lineage relationship to conventional T cells is controversial. We show here that the development of NKT cells is absolutely dependent on expression of the pre-TCRalpha chain, in marked contrast to conventional T cells which arise in significant numbers even in the absence of a functional pre-TCR. Distinct developmental requirements for pre-TCR expression in the NKT and T cell lineages may reflect differences in the ability of the TCRalphabeta to substitute functionally for the pre-TCR in immature precursor cells.
Animals, Cell Differentiation, Female, Hematopoietic Stem Cells/cytology, Hematopoietic Stem Cells/immunology, Killer Cells, Natural/cytology, Killer Cells, Natural/immunology, Mice, Mice, Inbred C57BL, Mice, Knockout, Receptors, Antigen, T-Cell, alpha-beta/genetics, Receptors, Antigen, T-Cell, alpha-beta/metabolism, Receptors, Antigen, T-Cell, gamma-delta/genetics, Receptors, Antigen, T-Cell, gamma-delta/metabolism, T-Lymphocytes/cytology, T-Lymphocytes/immunology
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