The cloned RNA polymerase II transcription factor IID selects RNA polymerase III to transcribe the human U6 gene in vitro.

Détails

ID Serval
serval:BIB_0F82DC47A43D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The cloned RNA polymerase II transcription factor IID selects RNA polymerase III to transcribe the human U6 gene in vitro.
Périodique
Genes and Development
Auteur(s)
Lobo S.M., Lister J., Sullivan M.L., Hernandez N.
ISSN
0890-9369[print], 0890-9369[linking]
Statut éditorial
Publié
Date de publication
08/1991
Volume
5
Numéro
8
Pages
1477-1489
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
Although the human U2 and U6 snRNA genes are transcribed by different RNA polymerases (i.e., RNA polymerases II and III, respectively), their promoters are very similar in structure. Both contain a proximal sequence element (PSE) and an octamer motif-containing enhancer, and these elements are interchangeable between the two promoters. The RNA polymerase III specificity of the U6 promoter is conferred by a single A/T-rich element located around position -25. Mutation of the A/T-rich region converts the U6 promoter into an RNA polymerase II promoter, whereas insertion of the A/T-rich region into the U2 promoter converts that promoter into an RNA polymerase III promoter. We show that this A/T-rich element can be replaced by a number of TATA boxes derived from mRNA promoters transcribed by RNA polymerase II with little effect on RNA polymerase III transcription. Furthermore, the cloned RNA polymerase II transcription factor TFIID both binds to the U6 A/T-rich region and directs accurate RNA polymerase III transcription in vitro. Mutations in the U6 A/T-rich region that convert the U6 promoter into an RNA polymerase II promoter also abolish TFIID binding. Together, these observations suggest that in the human snRNA promoters, unlike in mRNA promoters, binding of TFIID directs the assembly of RNA polymerase III transcription complexes, whereas the lack of TFIID binding results in the assembly of RNA polymerase II snRNA transcription complexes.
Mots-clé
Base Sequence, Cell Nucleus/physiology, Cloning, Molecular, Hela Cells/physiology, Humans, Models, Genetic, Molecular Sequence Data, Mutagenesis, Site-Directed, Promoter Regions, Genetic, RNA Polymerase II/metabolism, RNA Polymerase III/metabolism, RNA, Small Nuclear/genetics, Recombinant Proteins/metabolism, TATA Box, Transcription Factor TFIID, Transcription Factors/genetics, Transcription Factors/metabolism, Transcription, Genetic, Transfection
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2008 16:33
Dernière modification de la notice
20/08/2019 12:36
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