Epithelial Sodium Channel-Mediated Sodium Transport Is Not Dependent on the Membrane-Bound Serine Protease CAP2/Tmprss4.

Détails

Ressource 1Télécharger: BIB_0EC60EC08C02.P001.pdf (2160.97 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_0EC60EC08C02
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Epithelial Sodium Channel-Mediated Sodium Transport Is Not Dependent on the Membrane-Bound Serine Protease CAP2/Tmprss4.
Périodique
Plos One
Auteur(s)
Keppner A., Andreasen D., Mérillat A.M., Bapst J., Ansermet C., Wang Q., Maillard M., Malsure S., Nobile A., Hummler E.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
10
Numéro
8
Pages
e0135224
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
The membrane-bound serine protease CAP2/Tmprss4 has been previously identified in vitro as a positive regulator of the epithelial sodium channel (ENaC). To study its in vivo implication in ENaC-mediated sodium absorption, we generated a knockout mouse model for CAP2/Tmprss4. Mice deficient in CAP2/Tmprss4 were viable, fertile, and did not show any obvious histological abnormalities. Unexpectedly, when challenged with sodium-deficient diet, these mice did not develop any impairment in renal sodium handling as evidenced by normal plasma and urinary sodium and potassium electrolytes, as well as normal aldosterone levels. Despite minor alterations in ENaC mRNA expression, we found no evidence for altered proteolytic cleavage of ENaC subunits. In consequence, ENaC activity, as monitored by the amiloride-sensitive rectal potential difference (ΔPD), was not altered even under dietary sodium restriction. In summary, ENaC-mediated sodium balance is not affected by lack of CAP2/Tmprss4 expression and thus, does not seem to directly control ENaC expression and activity in vivo.
Mots-clé
Absorption, Physicochemical, Animals, Biological Transport, Cell Membrane/metabolism, Epithelial Sodium Channels/metabolism, Gene Knockout Techniques, Homeostasis, Membrane Proteins/deficiency, Membrane Proteins/genetics, Mice, Serine Endopeptidases/deficiency, Serine Endopeptidases/genetics, Sodium/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
27/08/2015 13:35
Dernière modification de la notice
20/08/2019 12:35
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