Ascorbate modulation of bovine chondrocyte growth, matrix protein gene expression and synthesis in three-dimensional collagen sponges.

Détails

ID Serval
serval:BIB_0EB639DF4E7F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Ascorbate modulation of bovine chondrocyte growth, matrix protein gene expression and synthesis in three-dimensional collagen sponges.
Périodique
Biomaterials
Auteur(s)
Ronzière M.C., Roche S., Gouttenoire J., Démarteau O., Herbage D., Freyria A.M.
ISSN
0142-9612 (Print)
ISSN-L
0142-9612
Statut éditorial
Publié
Date de publication
2003
Volume
24
Numéro
5
Pages
851-861
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
This report completes a previous study on the growth and metabolism of fetal bovine epiphyseal chondrocytes cultured, within native or cross-linked collagen sponges carried out without the addition of fresh ascorbate. At low initial cell density (2.3 x 10(6)cells/cm(3)) cell proliferation and a low matrix deposition were observed, whereas at high initial cell density (2.3 x 10(7)cells/cm(3)) there was an absence of cell proliferation, but the deposition of a cartilage-like matrix was measured. In both cases, only traces of type I collagen (marker of chondrocyte dedifferentiation) were detected. In this report, we observed, after 1 month in culture with ascorbate, in both type of scaffolds and initial cell densities, an increase in cell proliferation (2-fold) and in expression of genes encoding for collagen types I, II, X and MMP-2 and -13, but no change in the level of matrix deposition (collagen and GAG). With regard to the proteins present, the main differences with or without ascorbate concerned the increase of neosynthesised type I collagen (up to 35% of the total collagen deposited in the sponge) and of the MMP-2 active form. In conclusion, these results show that ascorbate is an important factor to consider when preparing cartilage constructs for its action on chondrocyte phenotype modulation and proliferation.
Mots-clé
Animals, Ascorbic Acid/pharmacology, Base Sequence, Cattle, Cell Division/drug effects, Cells, Cultured, Chondrocytes/cytology, Chondrocytes/drug effects, Collagen/genetics, DNA Primers, Extracellular Matrix Proteins/genetics, Gelatinases/genetics, Gene Expression Regulation/drug effects, Gene Expression Regulation, Enzymologic/drug effects, Glycosaminoglycans/genetics, Immunohistochemistry, Kinetics, Reverse Transcriptase Polymerase Chain Reaction, Time Factors
Pubmed
Création de la notice
01/10/2015 16:23
Dernière modification de la notice
20/08/2019 13:35
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