Article: article d'un périodique ou d'un magazine.
Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis.
Lab on a Chip
Date de publication
Publication types: Journal Article Publication Status: ppublish
A thin chip polymer-based microsprayer has been coupled to a hybrid quadrupole time-of-flight mass spectrometer (QTOF MS) and introduced in carbohydrate research. The feasibility of the approach is demonstrated for mapping, sequencing and structural elucidation of glycoconjugates originating from human body fluids and tissues such as a glycopeptide mixture from normal human urine and an isolated and purified GT1 ganglioside fraction from normal adult human brain. The optimization procedure required by each glycoconjugate category is described and the advantages of the system in terms of flexibility and adaptability to QTOF MS, stability of the ESI MS signal, carbohydrate ionization and sequencing, sensitivity, speed of analysis and sample consumption are discussed.
Amino Acids/analysis, Amino Acids/urine, Brain Chemistry, Cerebrospinal Fluid/chemistry, Gangliosides/analysis, Glycoconjugates/analysis, Glycoconjugates/urine, Glycopeptides/analysis, Glycopeptides/urine, Humans, Microchemistry/instrumentation, Microchemistry/methods, Microchip Analytical Procedures/instrumentation, Microchip Analytical Procedures/methods, Middle Aged, Nebulizers and Vaporizers, Reference Standards, Sensitivity and Specificity, Spectrometry, Mass, Electrospray Ionization/instrumentation
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