Species identification in mammals from mixed biological samples based on mitochondrial DNA control region length polymorphism.

Détails

ID Serval
serval:BIB_0E82259B54B5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Species identification in mammals from mixed biological samples based on mitochondrial DNA control region length polymorphism.
Périodique
Electrophoresis
Auteur(s)
Pun K.M., Albrecht C., Castella V., Fumagalli L.
ISSN
1522-2683[electronic]
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
30
Numéro
6
Pages
1008-1014
Langue
anglais
Résumé
The ability to identify the species origin of an unknown biological sample is relevant in the fields of human and wildlife forensics. However, the detection of several species mixed in the same sample still remains a challenge. We developed and tested a new approach for mammal DNA identification in mixtures of two or three species, based on the analysis of mitochondrial DNA control region interspecific length polymorphism followed by direct sequencing. Contrary to other published methods dealing with species mixtures, our protocol requires a single universal primer pair and is not based on a pre-defined panel of species. Amplicons can be separated either on agarose gels or using CE. The advantages and limitations of the assay are discussed under different conditions, such as variable template concentration, amplicon sizes and size difference among the amplicons present in the mixture. For the first time, this protocol provides a simple, reliable and flexible method for simultaneous identification of multiple mammalian species from mixtures, without any prior knowledge of the species involved.
Mots-clé
DNA mixtures, Length polymorphism, Mitochondrial DNA control region, Species identification, Wildlife forensics
Pubmed
Web of science
Création de la notice
04/08/2008 10:40
Dernière modification de la notice
29/01/2019 16:43
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