Characterisation of microbial communities colonising the hyphal surfaces of arbuscular mycorrhizal fungi.

Détails

ID Serval
serval:BIB_0DF7E6CB4E97
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Characterisation of microbial communities colonising the hyphal surfaces of arbuscular mycorrhizal fungi.
Périodique
ISME Journal
Auteur(s)
Scheublin T.R., Sanders I.R., Keel C., van der Meer J.R.
ISSN
1751-7370[electronic], 1751-7362[linking]
Statut éditorial
Publié
Date de publication
2010
Volume
4
Numéro
6
Pages
752-763
Langue
anglais
Résumé
Arbuscular mycorrhizal fungi (AMF) are symbiotic soil fungi that are intimately associated with the roots of the majority of land plants. They colonise the interior of the roots and the hyphae extend into the soil. It is well known that bacterial colonisation of the rhizosphere can be crucial for many pathogenic as well as symbiotic plant-microbe interactions. However, although bacteria colonising the extraradical AMF hyphae (the hyphosphere) might be equally important for AMF symbiosis, little is known regarding which bacterial species would colonise AMF hyphae. In this study, we investigated which bacterial communities might be associated with AMF hyphae. As bacterial-hyphal attachment is extremely difficult to study in situ, we designed a system to grow AMF hyphae of Glomus intraradices and Glomus proliferum and studied which bacteria separated from an agricultural soil specifically attach to the hyphae. Characterisation of attached and non-attached bacterial communities was performed using terminal restriction fragment length polymorphism and clone library sequencing of 16S ribosomal RNA (rRNA) gene fragments. For all experiments, the composition of hyphal attached bacterial communities was different from the non-attached communities, and was also different from bacterial communities that had attached to glass wool (a non-living substratum). Analysis of amplified 16S rRNA genes indicated that in particular bacteria from the family of Oxalobacteraceae were highly abundant on AMF hyphae, suggesting that they may have developed specific interactions with the fungi.
Mots-clé
arbuscular mycorrhizal fungi, bacterial community, hyphae, plant-microbe interactions, rhizosphere, T-RFLP
Pubmed
Web of science
Open Access
Oui
Création de la notice
05/01/2010 16:19
Dernière modification de la notice
08/05/2019 14:24
Données d'usage